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KMID : 0545120090190101197
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 10 p.1197 ~ p.1200
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Purification and characterization of neoagarotetraose from hydrolyzed agar
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Jang Min-Kyung
Lee Dong-Guen
Kim Nam-Young
Yu Ki-Hwan
Jang Hye-Ji
Lee Seung-Woo
Jang Hyo-Jung
Lee Ye-Ji
Lee Sang-Hyeon
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Abstract
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Whitening effect, tyrosinase inhibitions, and cytotoxicity of neoagarotetraose were measured after purification from hydrolyzed agar by gel filtration chromatography. In melanoma B16F10 cells, melanin content of neoagarotetraose-treated cells was as same as that treated by kojic acid or arbutin. In addition, tyrosinase of melanoma cells was strongly inhibited by neoagarotetraose at a concentration of 1 μg/ml and similarly inhibited at 10 and 100 μg/ml compared to those by arbutin or kojic acid. The activity of mushroom tyrosinase showed a 38% inhibition by neoagarotetraose at 1 μg/ml, and this inhibitory effect was more efficient than that by kojic acid. Neoagarotetraose revealed similar IC50 (50% inhibition concentration) value for mushroom tyrosinase as that by kojic acid. These data suggest that neoagarotetraose generated from agar by recombinant β-agarase might be a good candidate as a cosmetic additive for whitening effect.
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KEYWORD
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beta-agarase, neoagarotetraose, neoagarohexaose, neoagarooligosaccharides, whitening effect, mushroom tyrosinase
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